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By Gerald H. Wagman and Marvin J. Weinstein (Eds.)

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Randall, Assay Methods of Antibiotics. , New York, 1955. H. M. J. Weinstein, Appl. , 16 (1968) 624-627. C. -P. Rosselet, Proc. Soc. Expt. Biol. , 104 (1960) 304-306. 5 R. Dulbecco, Proc. Nut. Acad. Sci. , 38 (1952) 747. 6 E. A. Smith, J. , 14 (1964) 129-132. 7 E. C. Erickson, J . , 26 (1967) 531-532. J. M. Kline, J. , 64 (1972) 182-184. 9 E. A. Chang, J. , 32 (1979) 846-848. 10 N. Narasimhachari and S. Ramachandran, J. , 27 (1967) 494. B. E. Cook, J. , 35 (1968) 295-296. L. Homans and A. Fuchs, J.

Hamada. K. Maeda. Okami and H. Umezawa, J . , 21 (1967) 37 ELPHO Medium Whatman No. 3MM paper TLC 1 Medium Silica gel 60 F254(E. Merck) Solvents A. Chloroform-methanol (20: 1) B. Acetone-n-hexane ( 1 : 1) RF ~~ Buffer Formic acid-acetic acid-water (25 :75 :900) Conditions 3000 V, 100- 150 mA for 30 min Detection Ninhydrin. UV absorption; biological activity Ref. As PC ACIDOPHILIN TLC Media A. Silica gel B. Alumina Solvent Chloroform-methanol (90: 10) Detection A. U V B. H 2S04 charring C. 38 Re/.

R. Remers and R. Grulich, J. , 0s-3256) Detection Biological activity RF PC Solvent Ascending method. 45-ca. 50 Ref T. Hata, I. Umezawa, Y. Iwai, M. Katagiri, J. Awaya, K. Komiyama, R. Oiwa and K. Atsumi, J. 04 F G Ref: As PC PC Solvents A. Wet butanol B. Aq. ammonium chloride 3% C. Aq. ammonium chloride 20% D. Aq. acetone E. Butanol-methanol-water (4: 1 :2) F. Benzene-methanol (4: 1) G. 67 E Re{ N. Bagahama, M. Suzuki, S. Awataguchi and T. Okuda, J. , 20 (1957) 261-266 TLC 2 Medium Silica gel Uniplate, Analtech, Inc.

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