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Additional info for B Vitamins and Folate: Chemistry, Analysis, Function and Effects
In 1927, following the crystallization of the antineuritic vitamin, the British Committee on Accessory Food Factors distinguished the heat-labile and heat-stable components of vitamin B, and named the former B1 and the latter B2. In 1933 Richard Kuhn, Paul Gyo¨rgy and Theodor Wagner-Jauregg isolated from egg white a yellow pigment having vitamin B2 activity, which they crystallized and named ‘ovo-ﬂavin’ (Kuhn et al. 1933). They noticed that the absorption spectra of ovo-ﬂavin resembled those of the ‘yellow enzyme’ isolated by Warburg and Christian (see below).
All six forms have vitamin B6 activity; hence they are called B6 vitamers. Two of the phosphorylated forms, pyridoxal phosphate and pyridoxamine phosphate, act as coenzymes. Pyridoxine phosphate is considered to be the intermediate on the way from pyridoxine to pyridoxal phosphate. While developing a microbiological assay system for pyridoxine, Snell noticed that the content of ‘pyridoxine’ in rat tissues as determined by growth of Streptococcus faecalis was several thousand times higher than those obtained by yeast growth, rat growth or colorimetric methods.
Harris and Folkers and Kuhn and associates in 1939 (Gyo¨rgy 1964). 5). 5 OH HO N Pyridoxine (Pyridoxol) OH HO P O O NH2 O Pyridoxamine NH2 OH HO P O O OH N Pyridoxamine phosphate B6 vitamers. All six forms have vitamin B6 activity; hence they are called B6 vitamers. Two of the phosphorylated forms, pyridoxal phosphate and pyridoxamine phosphate, act as coenzymes. Pyridoxine phosphate is considered to be the intermediate on the way from pyridoxine to pyridoxal phosphate. While developing a microbiological assay system for pyridoxine, Snell noticed that the content of ‘pyridoxine’ in rat tissues as determined by growth of Streptococcus faecalis was several thousand times higher than those obtained by yeast growth, rat growth or colorimetric methods.