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By Amitava Dasgupta

Antioxidants in meals, supplements and Supplements bridges the distance among books geared toward shoppers and technical volumes written for investigators in antioxidant learn. It explores the position of oxidative rigidity within the pathophysiology of varied ailments in addition to antioxidant meals, supplementations, and all antioxidant vitamins, together with natural supplementations. It deals healthcare execs a wealthy source of key scientific details and easy medical causes correct to the advance and prevention of particular illnesses. The ebook is written at an intermediate point, and will be simply understood via readers with a faculty point chemistry and biology background.

  • Covers either oxidative stress-induced illnesses in addition to antioxidant-rich meals (not the chemistry of antioxidants)
  • Contains easy-to-read tables and figures for fast reference details on antioxidant meals and vitamins
  • Includes a glycemic index and a desk of ORAC values of varied vegatables and fruits for clinicians to simply make suggestions to patients

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33] Brigelius-Flohe R, Mariorino M. Glutathione peroxidases. Biochim Biophys Acta 2013;830:3289À303. [34] Kalyanaraman B. Teaching the basics of redox biology to medical students and graduate students: oxidants, antioxidants and disease mechanisms. Redox Biol 2013;8:244À57. [35] Poynton RA, Hampton MB. Peroxiredoxins as biomarker of oxidative stress. Biochim Biophys Acta 2013 [E-pub ahead of print]. [36] Kondo N, Nakamura H, Masutani H, Yodoi J. Redox regulation of human thioredoxin network. Antioxid Redox Signal 2006;8:1881À90.

Oxidation of RNA produces 8-oxo-7,8-dihydroguanosine (8-oxoGuo), and the urine level of this marker is often used for investigating in vivo oxidative stress. This marker, along with 8-oxodG, can be measured by ultra performance liquid chromatography combined with tandem mass spectrometry [49]. 6 MEASUREMENT OF ANTIOXIDANT ENZYMES AND OTHER COMPOUNDS For analysis of common antioxidant enzyme such as superoxide dismutase, glutathione peroxidase, glutathione reductase, and catalase, several methods have been described in the literature.

However, FRAP assay can also be carried out using ferricyanide ● 25 26 CHAPTER 2: M e t ho ds f o r M ea s ur i ng O x i da t iv e S t r e s s i n t h e L a b o r a t o r y [Fe31 (CN2)6], in which an antioxidant donates an electron and converts ferricyanide to ferrocyanide [Fe21 (CN2)6], which in the presence of excess ferric ion (ferric chloride) produces an intense blue color (Perl’s Prussian blue). The intensity of the color can be measured at 700 nm [17]. Cupric ion reducing power of an antioxidant can also be used to measure total antioxidant capacity of a compound.

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